Total Ionizing Dose(TID) radiation-hard technology in Shallow Trench Isolation(STI) is developed based on 0.18 μm CMOS process,which raises the threshold of P-type silicon at the STI/ substrate interface by ion implantation technology, and therefore enhances the radiation hardening ability of NMOS transistors. There is no threshold shift of the NMOS transistors after irradiating at 500 krad(Si). The leakage current keeps at the order of magnitude of 10-12. It has better radiation hardening characteristic compared with the NMOS transistors without radiation-hard. Through the study of TID radiation-hard technology in STI, the radiation hardening ability of circuit can be enhanced. At the same time, this technology will avoid the problem that the area of chip will increase due to the radiation-hard design.