An Ultra-Wideband(UWB) Multiple-Input Multiple-Output(MIMO) antenna improving isolation degree is designed and discussed. Two basic antenna units in diagonal layout are located at the other side of dielectric-slab. A kind of V-shaped coupler with the style of fence is introduced to reduce the coupling between antenna units. The working frequency band of the MIMO antenna is from 3.3 GHz to 12.02 GHz. The proposed MIMO antenna meets the requirements on isolation and diversity gain through simulation and testing.