The I-U characteristic curves of Dynamic Threshold MOS(DTMOS) transistor are obtained based on 0.5 μm Complementary Metal Oxide Semiconductor(CMOS) process. Compared with the PNP transistor of conventional CMOS technology, the design criteria are given, which are used to design the radiation-hardened CMOS reference. The radiation-hardened CMOS reference is completed by using DTMOS diodes as radiation tolerance diodes and combining the Radiation Hardening By Design(RHBD) technology. The experimental results show that the ability of anti-γ ionizing radiation total dose reaches up to 300 krad(Si) for the radiation-hardened CMOS reference.