基于太赫兹光谱的胶质瘤异质性表征

Characterization of glioma heterogeneity based on terahertz spectroscopy

摘要:

通过揭示胶质瘤与对侧正常脑组织的THz光谱特征，分析肿瘤空间的光谱差异，为无创肿瘤诊断提供理论支持。采用U87胶质瘤细胞原位荷瘤小鼠模型，利用太赫兹光谱技术，表征胶质瘤病灶区域及对侧脑组织的吸收特性。采用单因素方差分析(ANOVA)和Tukey's HSD事后检验，评估肿瘤不同层次间光谱吸收的显著性差异。免疫荧光结果显示，胶质瘤病灶区域细胞增殖能力和血管生成密度具有差异性。THz光谱分析表明，在2 THz以上，肿瘤区域的吸收系数显著高于正常脑组织，尤其是外周周围区(L(6-7))吸收系数高于肿瘤增强区(L(1-2))。ANOVA分析验证了肿瘤不同层次间的光谱吸收差异具有统计学显著性(p<0.05)，Tukey's HSD检验进一步确认了肿瘤内部各层次间的具体差异。方差齐性检验显示，肿瘤内部各层次存在显著的异质性，而正常脑组织区域则显示出较为一致的光谱特征。研究表明太赫兹光谱技术可有效识别胶质瘤内部异质性，尤其是病灶中心和浸润区的吸收差异，为无创肿瘤诊断提供了重要依据，展示了其应用潜力。

Abstract:

By revealing the THz spectral characteristics of glioma and contralateral normal brain tissue, and analyzing the spectral differences in tumor space, this study provides theoretical support for non-invasive tumor diagnosis. Using an orthotopic U87 glioma cell tumor-bearing mouse model, terahertz spectroscopy is employed to characterize the absorption properties of the glioma lesion area and the contralateral brain tissue. Single-factor Analysis Of Variance(ANOVA) and Tukey's Honestly Significant Difference(HSD) post hoc test are adopted to assess the significant differences in spectral absorption between different layers of the tumor. Immunofluorescence results show differences in cell proliferation ability and vascular density in the glioma lesion area. THz spectral analysis indicates that above 2 THz, the absorption coefficient of the tumor area is significantly higher than that of normal brain tissue, especially with the peripheral surrounding area (L(6-7)) having a higher absorption coefficient than the tumor enhancement area (L(1-2)). ANOVA analysis confirms that the spectral absorption differences between different layers of the tumor are statistically significant(p<0.05), and Tukey's HSD test further confirms the specific differences between each layer within the tumor. Homogeneity of variance test shows significant heterogeneity within the tumor layers, while the normal brain tissue area exhibits more consistent spectral characteristics. The study demonstrates that terahertz spectroscopy can effectively identify the internal heterogeneity of glioma, especially the absorption differences between the lesion center and the infiltration area, providing important evidence for noninvasive tumor diagnosis and showcasing its application potential.